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We saw that friction related effects were more important for small molecule separations whilst pressure effects were much more significant for protein separations. Insulin was an attractive example kakım we could clearly see the decrease of retention in constant inlet pressure mode and the increase of retention in variable inlet pressure mode when increasing the flow rate.
We hope that this information will make the life of practicing chromatographers easier! In the common practice of analytical R&D laboratories located in most biopharmaceutical companies, when developing a new method, several method variables are typically screened over a wide range. This is time consuming and above all unnecessary in many cases.
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This article describes the here analytical challenges of chemical exposomics in food safety. The need for high-throughput, multi-ortam approaches—such bey LC–HRMS and GC–HRMS with IMS—to capture the full spectrum of potential contaminants in our food supply is emphasized.
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